13
Nov

Cellulars

Almost the one year in the region of ' ' Cracolndia' ' , I had the displeasure of walking in a police viatura, in a search (frustrated) for a cellular one that he had been pulled out of me with a knife rusted in a lighthouse. This stroll placed me front to a reality that only knew for the TV, children, old, young adult becoming vacant made buzzes, people agglomerations that if wasted done cloud of grasshoppers to the measure that the car of polices if it approached Vi, (because to know I already wise person) that the Crack does not choose age and nor social classroom and the robery of cellular pra me started to be a chateao and not a problem. This everything pra to say that today I heard in the radio on a curious event of the CULTURAL TURN THAT HAPPENS Every year IN SO PAULO and I was surprised. It would be fact, a nocturnal stroll with lanterns, in the region of the Cracolndia! Therefore he is, I also scared and thought ' ' he is clearly that those people who vi will not be there, the city hall will be puted in charge of retir-las' ' That region is the shame of the city, perhaps ours bigger example of incivilidade, urban degradation and human being. But on the other hand: Because this must be hidden? Because we are that we have that arresting in them in the houses and of preference in closed condominiums, intimidated for the moral and urban violence. If to look at for this side would be an excellent chance population to say it that: It wants yes to walk in the streets (in any one) that this type of degradation wounds our right to go and to come. Who also knows is not an excellent same chance that of emblematic or innocent form to take light that place, a chance of appropriation of the space for more chaotic than either. Who knows our governing, mainly the municipal theatres, exactly that at a brief moment of lucidity they perceive that exists much people more than (and people are synonymous of votes in cabecinha of them) and what they think interested about this revitalizao. Mi40NC4wLjA’>Bobby Sharma.

What it happens in that place is immoral, not only for the Crack, but because there an invisible order of forbidden exists to pass. I know that we are ahead of something much more complex of what the project of a building, and that the urban universe if extends very beyond even though what ' ' urbanismo' ' it obtains to reach. But at least as architects and City planners we are called however or other to assume our roles or at least thinking about them They are initiatives as this that brings a little of light (exactly that of lanterns) for our lack of hope As &#039 says Italo Calvin in Invisible Cities; ' The hell of the livings creature is not something that will be; if it exists, is that one that already is here, the hell in which we live every day, that we form being together. Two ways exist not to suffer. The first one is easy for the majority of the people: to accept the hell and to become part of this until the point to leave to perceive it. Second she is risky and it demands attention and continued learning: to try to know to recognize who and what, in the way it hell, is not hell, and to preserve it and to open espao' ' Let us think about this!

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This method of transformation is constituted of an accelerator (also called cannon) that I impelled metallic micron-projectiles (particles) loading DNA for inside of unbroken cells, fabrics or vegetal agencies, that later are regenerated (BESPALHOK F., et al WAR; OLIVEIRA, 2000). Some models of accelerators exist, but the most used currently they use the gas helium compressed to generate the necessary force for the particle acceleration (SANFORD et al., 1991). It enters the used archetypes more we have ‘ ‘ Biolistic? PDS 1000/He’ ‘ of the BioRad company who uses high helium pressures. The used metallic particles more in the bombardeamento are of tungsten and the gold. (BESPALHOK F., et al WAR; OLIVEIRAS, 2000) the particles are prepared with the precipitation of the DNA.

The used method more for DNA precipitation uses CCl2 and espermidina associated with tungsten particles. The transformation for bombardeamento occurs under vacuum, to increase the efficiency of penetration of particles (SANFORD et al., 1991). By this method several had been transformed species such as soy and maize. The bombardeamento of particles also has been used for the transformation of cloroplastos and mitocndrias (BESPALHOK F., et al WAR; OLIVEIRA, 2000). Markers of election are necessary to increase the transgnicas production of cells and plants.

An election marker allows the preferential growth of the cells transformed into the presence of the selective agent, preventing the growth of the cells not transformed. Genes that confer resistance the antibiotics or herbicidas can be used as marking of election. (BESPALHOK F., et al WAR; OLIVEIRA, 2000). Genes of resistance the herbicidas also have been used as frequently marking of election. It enters most used, we have the gene BAR that confers resistance to the herbicida Basta (fosfonotricina active principle or PPT) (BESPALHOK F., et al WAR; OLIVEIRA, 2000). For each protocol of transformation, it is necessary to determine the dose of the adjusted selective agent for the used species and cellular type.

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